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RNA And DNA Extraction Kit , RNA / DNA Purification Micro Kit 20preps 50preps

Categories DNA Extraction Kit
Brand Name: Bioteke
Model Number: RNA/DNA Purification Micro Kit (Spin-column)
Certification: CE
Place of Origin: Beijing&Wuxi,China
MOQ: 100 boxes
Packaging Details: standard carton box package
DNA Extraction kit: RNA/DNA Purification Micro Kit (Spin-column)
DNA Isolation Kit: RNA/DNA Purification Micro Kit (Spin-column)
DNA Purification Kit: RNA/DNA Purification Micro Kit (Spin-column)
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BioTeke Corporation
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    RNA And DNA Extraction Kit , RNA / DNA Purification Micro Kit 20preps 50preps

    RNA/DNA Purification Micro Kit (Spin-column)


    Offered in two sizes (20preps, 50preps), DP3301,DP3302


    Bioteke’s RNA/DNA Purification Micro Kit provides a rapid method for the isolation and purification of total RNA and genomic DNA sequentially from a single sample of cultured animal cells, tissue samples (particularly hard-to-extract fibrous tissues) or blood. The total RNA and genomic DNA are both column purified in less than 30 minutes. The purified RNA and DNA fractions can be eluted in as little as 20 L. This kit is ideal for researchers who are interested in studying the genome and transcriptome of a single sample, such as for studies of microRNA profiling, gene expression including gene silencing experiments or mRNA knockdowns, studies involving biomarker discovery, and for characterization of cultured cell lines. Bioteke’s RNA/DNA Purification Micro Kit is especially useful for researchers who are isolating macromolecules from precious, difficult to obtain or small samples such as biopsy materials or single foci from cell cultures, as it eliminates the need to fractionate the sample. Furthermore, analysis will be more reliable since the RNA and DNA are derived from the same sample, thereby eliminating inconsistent results. The purified macromolecules are of the highest purity and can be used in a number of different downstream applications.


    Bioteke’s Purification Technology

    Purification is based on spin column chromatography. The process involves first lysing the cells or tissue of interest with the provided Buffer SKP. For hard-to-extract fibrous tissues (such as heart and muscle), an additional Proteinase K digestion step is provided for maximum recovery of the RNA and DNA. The DNA in the lysate is then captured and purified on a gDNA Purification Micro Column. Ethanol is then added to the flowthrough of the DNA purification step, and the solution is loaded onto an RNA Purification Micro Column. Bioteke’s resin binds nucleic acids in a manner that depends on ionic concentrations, thus only the RNA including microRNAs will bind to the column while the proteins are removed in the flowthrough. Next, the bound RNA is washed with the provided Wash Solution A to remove impurities, and the purified RNA is eluted with the Elution Solution A. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). Both RNA and DNA fractions can be eluted in as little as 20 L, resulting in a higher concentration of nucleic acids. The purified RNA is of the highest integrity and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays. The genomic DNA is of the highest quality, and can be used in PCR reactions, sequencing, Southern blotting, methylation studies and SNP analysis.


    Advantages

    • The RNA and DNA are both column purified

    • Both the RNA and DNA can be eluted in as little as 20 L

    • RNA and DNA are isolated from a single sample with no splitting of the lysate, thus

      reducing inconsistent results and variability

    • Sequential isolation of RNA and DNA from a single sample. Ideal for precious, difficult to

      obtain or small samples such as biopsy material or single foci from cell cultures.

    • Isolate total RNA and genomic DNA from a single sample in less than 30 minutes

    • Additional Proteinase K provided for hard-to-extract fibrous tissues.

    • All sizes of RNA are isolated, from large mRNA down to microRNA

    • The purified RNA and DNA are of the highest quality and can be used in a number of

      downstream applications

    Storage Conditions and Product Stability

    Store Proteinase K at -20°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 2 years in their unopened containers.


    Precautions and Disclaimers

    This kit is designed for research purposes only. It is not intended for human or diagnostic use. Ensure that a suitable lab coat, disposable gloves and protective goggles are worn when working with chemicals.


    Buffer SKP contains guanidinium salts, and should be handled with care. Guanidinium
    salts form highly reactive compounds when combined with bleach, thus care must be taken to properly dispose of any of these solutions


    Blood of all human and animal subjects is considered potentially infectious. All necessary precautions recommended by the appropriate authorities in the country of use should be taken when working with whole blood.


    Customer-Supplied Reagents and Equipment

    You must have the following in order to use the RNA/DNA Purification Micro Kit:


    For All Protocols

    •  Benchtop microcentrifuge

    •  -mercaptoethanol (Optional)

    •  96 - 100 % ethanol

    •  Molecular biology grade water (Milli-Q® water)


    • For Animal Cell Protocols

    •  PBS (RNase-free)

    For Animal Tissue Protocol

     Liquid nitrogen

    Mortar and pestle

      1. Working with RNA

        RNases are very stable and robust enzymes that degrade RNA. Autoclaving solutions and glassware is not always sufficient to actively remove these enzymes. The first step when preparing to work with RNA is to create an RNase-free environment. The following precautions are recommended as your best defense against these enzymes.

        •  The RNA area should be located away from microbiological work stations

        •  Clean, disposable gloves should be worn at all times when handling reagents, samples,

          pipettes, disposable tubes, etc. It is recommended that gloves are changed frequently to

          avoid contamination

        •  There should be designated solutions, tips, tubes, lab coats, pipettes, etc. for RNA only

        •  All RNA solutions should be prepared using at least 0.05% DEPC-treated autoclaved

          water or molecular biology grade nuclease-free water

        •  Clean all surfaces with commercially available RNase decontamination solutions

        •  When working with purified RNA samples, ensure that they remain on ice during

          downstream applications


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