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Sequencing Grade TrypsinTrypsin SRT Sequencing Grade Enzyme

Categories Sequencing Grade Trypsin
Brand Name: YaxinBio
Model Number: SRT0202
Certification: ISO9001
Place of Origin: China
MOQ: 100ug
Payment Terms: T/T
Packaging Details: ice packaging
Delivery Time: 7 days
Price: 98$/100ug
Supply Ability: 1000g per week
Source: E. Coli
Purified by: HPLC
Physical form: Lyophilized powder
Specific activity: >4500 USP units/mg pro
Purity: One single main band at 24kD by SDS-PAGE
Contaminant activity: No chymotrypsin, carboxypeptidase A, or other protease contaminant.
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    Sequencing Grade TrypsinTrypsin SRT Sequencing Grade Enzyme

    Sequencing Grade TrypsinTrypsin SRT Sequencing Grade Enzyme


    Description


    Trypsin specifically hydrolyzes peptide bonds at the carboxyl side of lysine and arginine residues. Recombinant trypsin is free of any other proteases activities, and TPCK is unnecessary and not contained. Unmodified trypsin is subject to auto-proteolysis, generating fragments that can interfere

    with protein sequencing or HPLC/MS peptides analysis. YaxinBio’s sequencing grade modified trypsin

    is recombinant porcine trypsin modified by reductive methylation, rendering it resistant to proteolytic digestion.


    Main Features


    Format

    Liquid in 50mM HAc, or fluffy solid.

    Protein concentration

    0.5mg/ml in 50mM HAc

    Purity(RP-HPLC)

    ≥99% by HPLC

    Contaminant activity

    Recombinant porcine trypsin.

    No any other proteases activities contaminant.


    Unit Definition:One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a

    reaction volume of 3.0ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).


    Reconstitution


    Following reconstitution in 10 mM HCl, Trypsin, recombinant, proteomics grade is stable at -15 to

    -25 °C for up to one month. After first reconstitution the product must be stored in appropriate

    aliquots to avoid repeated freezing and thawing.


    Specificity


    Serine endopeptidase, hydrolyzing specifically proteins and peptides at the carboxy side of the

    basic amino acids Arg and Lys. Amide and ester bonds of Arg and Lys are also cleaved.

    Quality Sequencing Grade TrypsinTrypsin SRT Sequencing Grade Enzyme wholesale
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