Sequencing Grade TrypsinTrypsin SRT Sequencing Grade Enzyme
Trypsin specifically hydrolyzes peptide bonds at the carboxyl side
of lysine and arginine residues. Recombinant trypsin is free of any
other proteases activities, and TPCK is unnecessary and not
contained. Unmodified trypsin is subject to auto-proteolysis,
generating fragments that can interfere
with protein sequencing or HPLC/MS peptides analysis. YaxinBio’s
sequencing grade modified trypsin
is recombinant porcine trypsin modified by reductive methylation,
rendering it resistant to proteolytic digestion.
Liquid in 50mM HAc, or fluffy solid.
0.5mg/ml in 50mM HAc
≥99% by HPLC
Recombinant porcine trypsin.
No any other proteases activities contaminant.
Unit Definition:One USP unit of trypsin activity will produce a
Delta A253 of 0.003 per minute in a
reaction volume of 3.0ml at pH7.6 and 25℃, with BAEE as a substrate
(1cm light path).
Following reconstitution in 10 mM HCl, Trypsin, recombinant,
proteomics grade is stable at -15 to
-25 °C for up to one month. After first reconstitution the product
must be stored in appropriate
aliquots to avoid repeated freezing and thawing.
Serine endopeptidase, hydrolyzing specifically proteins and
peptides at the carboxy side of the
basic amino acids Arg and Lys. Amide and ester bonds of Arg and Lys
are also cleaved.